Genetic characterization and protective immunity of cold-adapted attenuated avian H9N2 influenza vaccine.
Identifieur interne : 001252 ( Main/Exploration ); précédent : 001251; suivant : 001253Genetic characterization and protective immunity of cold-adapted attenuated avian H9N2 influenza vaccine.
Auteurs : Joo Sub Lee [Corée du Sud] ; Hyun Soo Kim ; Sang Heui SeoSource :
- Vaccine [ 0264-410X ] ; 2008.
Descripteurs français
- KwdFr :
- Adaptation physiologique, Animaux, Basse température, Femelle, Grippe chez les oiseaux (), Grippe chez les oiseaux (immunologie), Grippe chez les oiseaux (virologie), Mutation, Oeufs (virologie), Poulets (immunologie), Poulets (virologie), Sous-type H9N2 du virus de la grippe A (immunologie), Substitution d'acide aminé, Tests d'inhibition de l'hémagglutination, Vaccins antigrippaux (génétique), Vaccins antigrippaux (immunologie), Vaccins atténués (génétique), Vaccins atténués (immunologie).
- MESH :
- génétique : Vaccins antigrippaux, Vaccins atténués.
- immunologie : Grippe chez les oiseaux, Poulets, Sous-type H9N2 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués.
- virologie : Grippe chez les oiseaux, Oeufs, Poulets.
- Adaptation physiologique, Animaux, Basse température, Femelle, Grippe chez les oiseaux, Mutation, Substitution d'acide aminé, Tests d'inhibition de l'hémagglutination.
English descriptors
- KwdEn :
- Adaptation, Physiological, Amino Acid Substitution, Animals, Chickens (immunology), Chickens (virology), Cold Temperature, Eggs (virology), Female, Hemagglutination Inhibition Tests, Influenza A Virus, H9N2 Subtype (immunology), Influenza Vaccines (genetics), Influenza Vaccines (immunology), Influenza in Birds (immunology), Influenza in Birds (prevention & control), Influenza in Birds (virology), Mutation, Vaccines, Attenuated (genetics), Vaccines, Attenuated (immunology).
- MESH :
- chemical , genetics : Influenza Vaccines, Vaccines, Attenuated.
- immunology : Chickens, Influenza A Virus, H9N2 Subtype, Influenza Vaccines, Influenza in Birds, Vaccines, Attenuated.
- prevention & control : Influenza in Birds.
- virology : Chickens, Eggs, Influenza in Birds.
- Adaptation, Physiological, Amino Acid Substitution, Animals, Cold Temperature, Female, Hemagglutination Inhibition Tests, Mutation.
Abstract
H9N2 influenza viruses are endemic in many Asian countries including China and Korea, and cause a considerable economic loss to chicken industry by reduction in egg production and about 30% mortality. Here we developed live cold-adapted attenuated H9N2 influenza vaccine by adaptation of viruses in hen's eggs at 25 degrees C. Genetic analysis shows that the cold-adapted H9N2 (A/Chicken/Korea/S1/03) viruses contain a total of 44 amino acid substitutions, of which 7 amino acids are identical to the loci identified in the cold-adapted H2N2 (A/Ann Arbor/6/60) vaccine strain compared to genes in wild-type H9N2 (A/Chicken/Korea/S1/03) influenza viruses. When cold-adapted H9N2 (A/Chicken/Korea/S1/03) influenza viruses were inoculated in layers viruses were detectable in the tracheas, not in the lungs, no reduction of egg production and mortality was observed in contrast to the infection of wild-type H9N2 influenza viruses, and CD8+ T lymphocytes expressing IFN-gamma were induced. When layers vaccinated with cold-adapted attenuated H9N2 (A/Chicken/Korea/S1/03) influenza viruses were challenged with wild-type H9N2 (A/Chicken/Korea/521/04) influenza viruses, they were protected from the loss of egg production and mortality. Our results suggest that cold-adapted attenuated H9N2 vaccine can be used for controlling the infection of H9N2 influenza viruses in chickens.
DOI: 10.1016/j.vaccine.2008.09.043
PubMed: 18838096
Affiliations:
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xml:lang="en"><term>Chickens</term><term>Eggs</term><term>Influenza in Birds</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term><term>Amino Acid Substitution</term><term>Animals</term><term>Cold Temperature</term><term>Female</term><term>Hemagglutination Inhibition Tests</term><term>Mutation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adaptation physiologique</term><term>Animaux</term><term>Basse température</term><term>Femelle</term><term>Grippe chez les oiseaux</term><term>Mutation</term><term>Substitution d'acide aminé</term><term>Tests d'inhibition de l'hémagglutination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">H9N2 influenza viruses are endemic in many Asian countries including China and Korea, and cause a considerable economic loss to chicken industry by reduction in egg production and about 30% mortality. Here we developed live cold-adapted attenuated H9N2 influenza vaccine by adaptation of viruses in hen's eggs at 25 degrees C. Genetic analysis shows that the cold-adapted H9N2 (A/Chicken/Korea/S1/03) viruses contain a total of 44 amino acid substitutions, of which 7 amino acids are identical to the loci identified in the cold-adapted H2N2 (A/Ann Arbor/6/60) vaccine strain compared to genes in wild-type H9N2 (A/Chicken/Korea/S1/03) influenza viruses. When cold-adapted H9N2 (A/Chicken/Korea/S1/03) influenza viruses were inoculated in layers viruses were detectable in the tracheas, not in the lungs, no reduction of egg production and mortality was observed in contrast to the infection of wild-type H9N2 influenza viruses, and CD8+ T lymphocytes expressing IFN-gamma were induced. When layers vaccinated with cold-adapted attenuated H9N2 (A/Chicken/Korea/S1/03) influenza viruses were challenged with wild-type H9N2 (A/Chicken/Korea/521/04) influenza viruses, they were protected from the loss of egg production and mortality. Our results suggest that cold-adapted attenuated H9N2 vaccine can be used for controlling the infection of H9N2 influenza viruses in chickens.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Kim, Hyun Soo" sort="Kim, Hyun Soo" uniqKey="Kim H" first="Hyun Soo" last="Kim">Hyun Soo Kim</name><name sortKey="Seo, Sang Heui" sort="Seo, Sang Heui" uniqKey="Seo S" first="Sang Heui" last="Seo">Sang Heui Seo</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Lee, Joo Sub" sort="Lee, Joo Sub" uniqKey="Lee J" first="Joo Sub" last="Lee">Joo Sub Lee</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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